[ effect of endurance training on MiR-155 expression, STAT3 gene expression, and interleukin 6 protein in mice with breast cancer]

Endurance training has an important role in the prevention and adjuvant therapy of breast cancer. The aim of the present study was to investigate the role of endurance training on miR-155 expression, signal transducer and activator of transcription-3 [STAT[3]] gene expression, and interleukin 6 [IL-6] protein in breast cancer tumor in mice. In this study, 16 female Balb/C mice were randomly divided into exercise-tumor [ET] and rest-tumor [RT] groups. The mice were oriented in the environment and one million estrogen-dependent breast cancer cells [MC4L2] were injected into each mouse. Subsequently, the ET group performed endurance exercise, 5 days per week for 6 weeks. Tumor volume was measured by a digital caliper weekly. Finally, the mice were sacrificed and tumor tissue was removed and kept in -70[degree]C. Then, RNA was extracted by the Trizol protocol and complementary DNA [cDNA] was synthesized according to guidelines of the Kit Company. Consequently, the real-time PCR method was performed and data was collected. Significant differences were observed between the ET and RT groups in the STAT[3] gene expression, miR-155 expression, and IL-6 protein [P < 0.05]. These results were consistent with tumor growth rate. Exercise can reduce miR-155 expression, STAT[3] gene expression, and IL-6 protein in tumor tissue. Due to the reduction in miR-155 expression, STAT[3] gene expression, and IL-6 protein in the ET group, it can be claimed that endurance training can be used as adjuvant therapy by decreasing of oncogenic and inflammation factors

Association between cardiovascular fitness and inflammatory markers in boys aged 11-14 years

It is reported that some inflammatory markers are predictive factors for atherosclerosis in childhood and adolescence and cardiovascular disease in adulthood. We investigated whether markers of inflammation including: cytokine [Interleukin-6 [IL-6]], acute-phase reactant [C-reactive protein [CRP]], white blood cell [WBC] count and its subgroups are associated with maximal oxygen consumption [VO[2max]] in overweight and normal children. Subjects were 26 boys aged 11-14 years included in two groups of overweight [n=10] and normal weight [n=16] children. VO[2max] was measured employing an incremental graded exercise test. IL-6 and CRP levels as well as WBC count were measured. Multivariable regression was employed to evaluate whether inflammatory markers were associated with VO[2max]. Mean VO[2max] for all subjects [n=26] was 36.35 +/- 10.42 ml/kg/min. This rate was lesser for overweight subjects [25.77 +/- 5.04] than in normal weight children [41.54 +/- 5.96]. Log IL-6, log CRP, and WBC count were correlated with VO[2max]. Also subgroups of WBC including Leukocytes, Lymphocyte, Neutrophils, Monocytes and Eosinophils associated with VO[2max]. IL-6, CRP and WBCs were inversely associated with aerobic or cardiorespiratory fitness levels measured by VO[2max] in children. This was independent of BMI of the subjects